Using a Spec 20 - State College of Florida, Manatee–Sarasota
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This was followed by a further addition of 0.1 ml 0.02% (w/v) phenolphthalein in 5 mM Na 2 CO 3, after which the reaction mixture was left to stand at room temperature for 15 min and the absorbance read at 550 nm. One unit of CGTase activity was defined as the amount of enzyme that liberated 1 μmol of β-cyclodextrin per min under the standard
